不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制研究     

李洁  杨静  赵丽丽 《武汉体育学院学报》2019,53(5):88-94

目的：探讨不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制。方法：将雄性Wistar大鼠50只随机均分为5组：安静对照组（C）、低负荷运动训练组（LT）、中等负荷运动训练组（MT）、高负荷运动训练组（HT）和极高负荷运动训练组（ST），每组10只。各运动组分别进行6周的跑台运动训练。训练方案结束后，取腓肠肌样本，提取线粒体，测定线粒体柠檬酸合成酶（CS）、异柠檬酸脱氢酶（ICD）和α-酮戊二酸脱氢酶（α-KGDHC）活性；线粒体Ga²⁺含量、胞浆NADH、NAD⁺、ATP和ADP含量，以及ICD mRNA转录水平。结果：（1）不同负荷运动训练组线粒体CS、ICD和α-KGDHC的活性均显著高于安静对照组（P < 0.01），且CS和ICD活性由高到低顺序均为：MT组 > HT组 > ST组 > LT组 > C组，α-KGDHC活性由高到低顺序为：HT组 > MT组 > ST组 > LT组 > C组。（2）不同负荷运动训练组线粒体Ca²⁺ 含量均显著高于安静对照组（P < 0.01），其含量由高到低顺序为：MT组 > HT组 > ST组 > LT组 > C组；胞浆NADH/NAD⁺和ATP/ADP的比值均显著低于安静对照组（P < 0.01），其比值由低到高顺序为：MT组 < ST组 < HT组 < LT组 < C组。（3）不同负荷运动训练组ICD mRNA转录水平均高于安静对照组（P < 0.01），其水平由高到低顺序为： MT组 > HT组 > ST组 > LT组 > C组。结论：低负荷、中等负荷、高负荷及极高负荷运动训练均可提高大鼠骨骼肌线粒体三羧酸循环功能，且中等负荷运动训练效果最佳。其机制与胞浆NADH/NAD⁺和ATP/ADP比值、线粒体摄钙能力及限速酶基因的表达有关。  相似文献   

集训对舞龙队女大学生血常规及内皮祖细胞的影响     

孔明涯  任路遥 《体育科技文献通报》2020,(4):156-158

目的:分析舞龙运动对女大学生血常规及内皮祖细胞含量的影响。方法:选取2017级舞龙队女大学生10人为训练组,2017级普通女大学生10人作为对照组。舞龙队女大学生备战比赛进行连续4周,每周3次,每次90min的赛前集训,普通女大学生除日常活动外,没有额外进行任何其他运动队的集训。集训结束后,在受试者都处于空腹状态下对两组受试者进行静脉采血并进行血常规、早期内皮祖细胞和晚期内皮祖细胞的检测。所得数据进行统计学分析。结果:参加舞龙集训的女大学生的血常规指标与普通女大学生的血常规指标无显著差异,早期EPCs和晚期EPCs无显著性差异,但在数值上显示出不同的趋势。结论:参加舞龙集训的女大学生身体处于健康状态。参加舞龙集训有可能增强运动员血管的自我修复能力。  相似文献   

Development of a chemically defined medium for Planctopirus limnophila to increase biomass production     

《Electronic Journal of Biotechnology》2021

BackgroundPlanctomycetes is a phylum of biofilm-forming bacteria with numerous biosynthetic gene clusters, offering a promising source of new bioactive secondary metabolites. However, the current generation of chemically defined media achieves only low biomass yields, hindering research on these species. We therefore developed a chemically defined medium for the model organism Planctopirus limnophila to increase biomass production.ResultsWe found that P. limnophila grows best with a 10 mM sodium phosphate buffer. The replacement of complex nitrogen sources with defined amino acid solutions did not inhibit growth. Screening for vitamin requirements revealed that only cyanocobalamin (B12) is needed for growth. We used response surface methodology to optimize the medium, resulting in concentrations of 10 g/L glucose, 34 mL/L Hutner’s basal salts, 23.18 mM KNO₃, 2.318 mM NH₄Cl and 0.02 mg/L cyanocobalamin. The analysis of amino acid consumption allowed us to develop a customized amino acid solution lacking six of the amino acids present in Aminoplasmal 10%. Fed-batch cultivation in a bioreactor using the optimized medium achieved a final ΔOD₆₀₀ of 46.8 ± 0.5 after 108 h, corresponding to a cell dry weight of 13.6 ± 0.7 g/L.ConclusionsThe optimized chemically defined medium allowed us to produce larger amounts of biomass more quickly than reported in earlier studies. Further research should focus on triggering P. limnophila biofilm formation to activate the gene clusters responsible for secondary metabolism.How to cite: Kruppa OC, Gerlach D, Fan R, et al. Development of a chemically defined medium for Planctopirus limnophila to increase biomass production. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.09.002.  相似文献   

Combined effects of exposure to hypoxia and cool on walking economy and muscle oxygenation profiles at tibialis anterior     

Masahiro Horiuchi  Yoko Handa-Kirihara  Daijiro Abe  Yoshiyuki Fukuoka 《Journal of sports sciences》2019,37(14):1638-1647

We investigated combined effects of ambient temperature (23°C or 13°C) and fraction of inspired oxygen (21%O₂ or 13%O₂) on energy cost of walking (C_w: J·kg^?1·km^?1) and economical speed (ES). Eighteen healthy young adults (11 males, seven females) walked at seven speeds from 0.67 to 1.67 m s^?1 (four min per stage). Environmental conditions were set; thermoneutral (N: 23°C) with normoxia (N: 21%O₂) = NN; 23°C (N) with hypoxia (H: 13%O₂) = NH; cool (C: 13°C) with 21%O₂ (N) = CN, and 13°C (C) with 13%O₂ (H) = CH. Muscle deoxygenation (HHb) and tissue O₂ saturation (StO₂) were measured at tibialis anterior. We found a significantly slower ES in NH (1.289 ± 0.091 m s^?1) and CH (1.275 ± 0.099 m s^?1) than in NN (1.334 ± 0.112 m s^?1) and CN (1.332 ± 0.104 m s^?1). Changes in HHb and StO₂ were related to the ES. These results suggested that the combined effects (exposure to hypoxia and cool) is nearly equal to exposure to hypoxia and cool individually. Specifically, acute moderate hypoxia slowed the ES by approx. 4%, but acute cool environment did not affect the ES. Further, HHb and StO₂ may partly account for an individual ES.  相似文献   

Effects of periodic carbohydrate ingestion on endurance and cognitive performances during a 40-km cycling time-trial under normobaric hypoxia in well-trained triathletes     

Yi-Hung Liao  Toby Mündel  Yan-Ting Yang  Chen-Chan Wei  Shiow-Chwen Tsai 《Journal of sports sciences》2019,37(16):1805-1815

The purpose of this study was to examine CHO ingestion on a cognitive task using a field-simulated time-trial (TT) under hypoxia in well-trained triathletes. Ten male triathletes (age: 22.1 ± 1.1 years; VO₂max: 59.4 ± 1.4 ml/kg/min) participated in this double-blind/crossover/counter-balanced design study. Participants completed 3 TT trials: 1) normoxic placebo (NPLA; FiO₂ = 20.9%), 2) hypoxic placebo (HPLA; FiO₂ = 16.3%), and 3) hypoxic CHO (HCHO; 6% CHO provided as 2 ml/kg/15 min; FiO₂ = 16.3%). During the TT, physiological responses (SpO₂, HR, RPE, and blood glucose/lactate), cognitive performance, and cerebral haemodynamics were measured. Hypoxia reduced TT performance by ~3.5–4% (p < 0.05), but CHO did not affect TT performance under hypoxia. For the cognitive task, CHO slightly preserved exercise-induced cognitive reaction speed but did not affect response accuracy during hypoxic exercise. However, CHO did not preserve the decreased Hb-Diff (cerebral blood flow, CBF) and increased HHb in the prefrontal lobe (p < 0.05) during hypoxic exercise, and CHO failed to preserve hypoxia-suppressed prefrontal CBF and tissue oxygen saturation. In conclusion, the present study demonstrates that CHO is effective in sustaining reaction speed for a cognitive task but not promoting TT performance during hypoxic exercise, which would be important for strategy-/decision-making when athletes compete at moderate high-altitude.  相似文献   

The effects of a calf pump device on second half performance of a simulated soccer match in competitive youth players     

S. Béliard  J. Cassirame  G. Ennequin  G. Coratella  N. Tordi 《Journal of sports sciences》2019,37(6):708-716

During soccer matches, performance decrements have been reported that relate to both physical abilities and technical skills. To investigate the effects of low-frequency electrical stimulation LFES (VeinoplusSport®, Ad Rem Technology, France) administered during half-time recovery on performance alterations during the second half. Twenty-two highly trained young players undertook a soccer-match simulation (SAFT⁹⁰). During half-time, they were randomly assigned to LFES group or Placebo group. Each half was split into 3 bouts of 12 minutes. Following each bout, maximal strike speed (MSS), sprint test (ST), maximal sprint accelerations (MA) and metabolic power (MP) were determined in both groups. Arterial (AF) and venous flows (VF) were measured at rest and at the end of half-time. LEFS group exhibited beneficial effects on performance compared to the Placebo group with a likely effect for MSS, ST, MA, and a possible effect for MP. AF and VF increased statistically more in LEFS group compared to Placebo group. The use of specific calf-pump LFES during half-time of a youth simulated soccer match attenuated the decrease in performance during the second half compared to Placebo group. This effect is most marked at the beginning of the second half with regards to explosive parameters.  相似文献   

低负荷加压训练改善自发性高血压大鼠的血压效果及机制研究     

谭朝文  赵彦  俞莹莹  马晓缓  郑玉婵 《体育科学》2020,(3):46-53,63

目的:探究低负荷加压训练对自发性高血压大鼠的降压效果及其作用机制。方法:选取4周龄雄性自发性高血压大鼠,随机分为对照组(高血压安静组)、低负荷训练组、低负荷加压训练组和高负荷训练组。低负荷训练组进行35%~55%1RM递进式低负荷爬梯训练,低负荷加压训练组进行30%~40%血流受限结合35%~55%1RM递进式低负荷爬梯训练,高负荷训练组进行55%~75%1RM递进式高负荷爬梯训练,训练后测定血压、血液中内皮素-1、血管内皮生长因子、一氧化氮合成酶的表达和心肌组织中内皮型一氧化氮合成酶的表达。结果:1)与对照组、高负荷训练组相比,低负荷加压训练组收缩压、舒张压显著下降(P<0.05);与低负荷训练组相比,低负荷加压训练组舒张压显著下降(P<0.05)。2)与对照组相比,低负荷加压训练组血液中内皮素-1表达显著下调(P<0.05),血管内皮生长因子和一氧化氮合成酶表达显著上调(P<0.05);3)与对照组相比,低负荷加压训练组心肌中内皮型一氧化氮合成酶表达显著上调(P<0.05);4)在低负荷加压训练组中,收缩压与内皮素-1呈正相关,相关性分析具有统计学意义(P<0.05);收缩压与一氧化氮合成酶、血管内皮生长因子、内皮型一氧化氮合成酶均呈负相关,相关性分析具有统计学意义(P<0.05)。结论:1)低负荷加压训练降压效果优于高负荷训练;2)低负荷加压训练能够通过下调血液中内皮素-1的表达,上调血液中血管内皮生长因子和一氧化氮合成酶的表达,同时上调心肌中内皮型一氧化氮合成酶表达,改善内皮细胞功能,达到降压的效果。  相似文献   

Wearable skin-like optoelectronic systems with suppression of motion artifacts for cuff-less continuous blood pressure monitor     

Haicheng Li  Yinji Ma  Ziwei Liang  Zhouheng Wang  Yu Cao  Yuan Xu  Hua Zhou  Bingwei Lu  Ying Chen  Zhiyuan Han  Shisheng Cai  Xue Feng 《国家科学评论(英文版)》2020,7(5):849

According to the statistics of the World Health Organization, an estimated 17.9 million people die from cardiovascular diseases each year, representing 31% of all global deaths. Continuous non-invasive arterial pressure (CNAP) is essential for the management of cardiovascular diseases. However, it is difficult to achieve long-term CNAP monitoring with the daily use of current devices due to irritation of the skin as well as the lack of motion artifacts suppression. Here, we report a high-performance skin-like optoelectronic system integrated with ultra-thin flexible circuits to monitor CNAP. We introduce a theoretical model via the virtual work principle for predicting the precise blood pressure and suppressing motion artifacts, and propose optical difference in the frequency domain for stable optical measurements in terms of skin-like devices. We compare the results with the blood pressure acquired by invasive (intra-arterial) blood pressure monitoring for >1500 min in total on 44 subjects in an intensive care unit. The maximum absolute errors of diastolic and systolic blood pressure were ±7/±10 mm Hg, respectively, in immobilized, and ±10/±14 mm Hg, respectively, in walking scenarios. These strategies provide advanced blood pressure monitoring techniques, which would directly address an unmet clinical need or daily use for a highly vulnerable population.  相似文献   

Acute cardiovascular responses to interval exercise: A systematic review and meta-analysis     

Kym Joanne Price  Brett Ashley Gordon  Stephen Richard Bird  Amanda Clare Benson 《Journal of sports sciences》2020,38(9):970-984

ABSTRACT

Interval exercise training is increasingly recommended to improve health and fitness; however, it is not known if cardiovascular risk is different from continuous exercise protocols. This systematic review with meta-analyses assessed the effect of a single bout of interval exercise on cardiovascular responses that indicate risk of cardiac fibrillation and infarction compared to continuous exercise. Electronic databases Medline, CINAHL, Embase, Scopus and Cochrane were searched. Key inclusion criteria were: (1) intervals of the same intensity and duration followed by a recovery period and (2) reporting at least one of blood pressure, heart rate variability, arterial stiffness or function. Cochrane Risk of Bias tool and GRADE approach were used. Meta-analyses found that systolic blood pressure responses to interval exercise did not differ from responses to continuous exercise immediately (MD 8 mmHg [95% CI ?32, 47], p = 0.71) or at 60 min following exercise (MD 0 mmHg [95% CI ?2, 1], p = 0.79). However, reductions in diastolic blood pressure and flow-mediated dilation with interval exercise were observed 10–15 min post-exercise. The available evidence indicates that interval exercise does not convey higher cardiovascular risk than continuous exercise. Further investigation is required to establish the safety of interval exercise for clinical populations.  相似文献   

Ferulic acid prevents liver injury and increases the anti-tumor effect of diosbulbin B in vivo   总被引：1，自引：0，他引：1  

Jun-ming WANG  Yu-chen SHENG  Li-li JI  Zheng-tao WANG 《Journal of Zhejiang University. Science. B》2014,15(6):540-547

The present study is designed to investigate the protection by ferulic acid against the hepatotoxicity induced by diosbulbin B and its possible mechanism, and further observe whether ferulic acid augments diosbulbin B-induced anti-tumor activity. The results show that ferulic acid decreases diosbulbin B-increased serum alanine transaminase/aspartate transaminase (ALT/AST) levels. Ferulic acid also decreases lipid peroxide (LPO) levels which are elevated in diosbulbin B-treated mice. Histological evaluation of the liver demonstrates hydropic degeneration in diosbulbin B-treated mice, while ferulic acid reverses this injury. Moreover, the activities of copper- and zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) are decreased in the livers of diosbulbin B-treated mice, while ferulic acid reverses these decreases. Further results demonstrate that the mRNA expressions of CuZn-SOD and CAT in diosbulbin B-treated mouse liver are significantly decreased, while ferulic acid prevents this decrease. In addition, ferulic acid also augments diosbulbin B-induced tumor growth inhibition compared with diosbulbin B alone. Taken together, the present study shows that ferulic acid prevents diosbulbin B-induced liver injury via ameliorating diosbulbin B-induced liver oxidative stress injury and augments diosbulbin B-induced anti-tumor activity.  相似文献